FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008178843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008178843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15652567
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA465890.29764446943431067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN278610.1779963631524465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCGGAAGAGCGTCGTGTAGGGAAAG204810.1308475472425705Illumina Single End PCR Primer 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187210.11960338518276267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)