FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008178847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008178847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15819800
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA473560.29934638870276487Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCGGAAGAGCGTCGTGTAGGGAAAG204810.12946434215350383Illumina Single End PCR Primer 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186050.1176057851553117No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN162990.10302911541233137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)