FastQCFastQC Report
Sat 2 Sep 2023
EGAF00008179287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008179287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322488786
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC271466198.417848985297741Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGAGATCGGAAGAGCACACG64623722.0039059590741863No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG60045191.8619310998305536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC49590631.537747424184852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGAGATCGGAAGAGCACAC43205691.3397579040159244No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA40280621.2490549051215691Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT33179461.0288562406011847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG30319850.9401830797304065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC26311060.8158751913934769No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA25306350.7847203096234174Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT24241710.7517070686606758No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC24122520.7480111261915321No Hit
TTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAAC24086020.7468793038899653Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT22555080.6994066454143308Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA20557070.6374506926265647Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA18316560.5679750985201699Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGAGATCGGAAGAGCACACGTC18039180.559373869204866No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG17294800.536291516195543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGAGATCGGAAGAGCACACGT15692570.4866082382163825No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT14280950.44283555335781505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACGTCTG13950990.43260387975165127Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC13870460.43010673865726295Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCCA13268260.4114332211229199Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTTGCGACCCGGGTTCGTTTCCCGGGCGGAGATCGGAAGAGCACACGT12978380.40244438143036704No Hit
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGA12812210.397291644119371Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAACT10716200.3322968259739736Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA10670920.3308927461434272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCTG9743220.3021258543855227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCAGATCGGAAGAGCACACGT9332250.28938215544648427No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG9061140.2809753514964083No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGATCGGAAGAGCACACGTCTGA8990980.27879977197098565Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACACG8904510.2761184384253287No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA8853780.27454536047030176Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC8754520.2714674239866437Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGTC8718650.27035513724809024No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTG8552890.265215113557468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG8361190.2592707208119789No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGAGATCGGAAGAGCACACGTC8031230.24903904720581507No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT7983260.2475515536220847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGAGATCGGAAGAGCACACGTCT7743430.24011470588003642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC7514770.23302422677109771No Hit
TTCTTGCGACCCGGGTTCGTTTCCCAGATCGGAAGAGCACACGTCTGAACT7214580.2237156860393899Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTCC7133760.22120955238424939Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGTC7125540.22095465980017057No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA7086110.21973198162617663Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC6857790.2126520455194991No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGT6637220.20581242784671588No Hit
CGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAA6625520.20544962453361093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC6484420.2010742785952253Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCAGATCGGAAGAGCACACG6456470.2002075817916968No Hit
TTTCTTGCGACCCGGGTTCGTTTCCCAGATCGGAAGAGCACACGTCTGAAC6341280.1966356746432727Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTC6314290.19579874631671687No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTGA6251550.19385325231122918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG6240910.19352331835811493Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG6124390.1899101694655516Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTTGCGACCCGGGTTCGTTTCCCGGGCGAGATCGGAAGAGCACACGTC6104670.18929867533440373No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTC6074280.1883563169852362No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG6069190.18819848203962045Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA6062700.1879972347317528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAACT5980290.18544179703662625Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGA5895400.18280945744265353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT5746500.1781922426288646No Hit
CCCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC5619310.1742482295182816No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACACGTCT5554570.17224071785243408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGAGATCGGAAGAGCACACGTCT5535600.17165248034392117Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT5493340.1703420471805181No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAAGATCGGAAGAGCACA5398840.16741171272851638No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACACGTCT5341150.16562281331543727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTGA5259370.16308691118332408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT5245260.1626493765894855No Hit
TCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG5224340.16200067186212175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA5192410.16101055991447716Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA5181440.16067039304740352Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT5011900.1554131559787012Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTC4991880.1547923591984994No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4875230.15117517915801265No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC4838050.15002227085192352No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG4793260.14863338534816525No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4741660.1470333297108818No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG4735550.14684386575848252Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACAC4687630.14535792261626115No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACAC4635080.14372840859030678No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC4562580.14148026840226313No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGAGATCGGAAGAGCACACGTCTGA4507740.13977974415519676Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA4349760.13488096916337425Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCTG4276850.13262011535495685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA4270460.13242196893010724Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT4265420.13226568442600048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG4192100.1299921169972093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC4191020.129958627460615Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT4114950.1275997857488291Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG4073770.12632284212202033Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGA4066270.12609027589567098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC4020010.12465580741154826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTAGTGTAGTGGTTATCACGTTCGAGATCGGAAGAGCACACGTCTGAA4017830.12458820816175604Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT3969750.12309730360670589No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG3950910.12251309724611634No Hit
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC3912380.12131832701928431Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA3876050.12019177621884812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC3843180.1191725159708344Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3839030.11904382932558777No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCAGATCGGAAGAGCACACGTC3803970.11795665973948007No Hit
GGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3802210.11790208419836341Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT3789700.1175141637328127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCT3776240.11709678487859108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGA3764410.11672995041756273Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAGATCGGAAGAGCACACGT3755560.11645552227047053No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC3700940.11476181996604373No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAGATCGGAAGAGCACAC3687190.11433544855106992No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT3682790.11419900969827831Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTG3679410.11409419985227022Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTGA3647370.11310067693330583Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCGTAGTGTAGTGGTTATCACGTTCGAGATCGGAAGAGCACACGTCTG3645070.11302935662389202Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACGTCTGA3642500.11294966393032965Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG3574470.11084013321319025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAAGATCGGAAGAGCACAC3511990.10890270150354933No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTCT3496590.10842516551877869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTC3460140.1072948936587209No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT3429450.10634323266049939No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC3389430.10510225927669932Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGAA3358220.10413447368678426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTGA3329790.10325289264476935Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGAA3290670.10203982720813119Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGT3290580.10203703641341502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)