FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008180670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008180670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32021037
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12454043.8893306297356953No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA6177421.9291754979702875No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT2545500.7949461474342633No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT1212750.3787353919862121No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT1053500.3290024617253964No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT763930.23857128674502326Clontech SMART CDS Primer II A (100% over 26bp)
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG657110.20521196737007613No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC563920.17610922469500284No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC506590.1582053698011092No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT486230.15184704980041713No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG482440.15066345290441405No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC466620.14572295082136158No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT423170.13215374630122068No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA381920.11927159011121345No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG372430.116307913450773No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)