FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15635176
Sequences flagged as poor quality0
Sequence length91
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11278777.213714767265811No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10553976.750144673779176No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9487136.067811452841976No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850131.822896013450696No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2143611.371017505655197No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1601911.0245551441186207No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1484800.9496535248467941No Hit
TCCCTTGCGATTTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1414290.9045564949188931No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1344180.8597152983759185No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1015310.6493754851240562No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1008720.6451606301073937No Hit
TCCCTTGCGATTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1006290.6436064422939659No Hit
TCCTAGTACCGAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA998900.6388799205074507No Hit
TCCCTTGCGATTTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990090.6332451902044467No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA985570.6303542729547784No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA958360.6129512069451601No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA902670.5773328039287822No Hit
CTCCCTAGTTCCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA888860.5685001563141983No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA836610.5350819204081873No Hit
GTATCCTTCCTTGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA827440.5292169400587495No Hit
TTCTTTGCCTTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA826630.5286988774542736No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT821870.5256544601736495No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA785240.5022265179490145No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA785210.502207330445145No Hit
TTCTTTGCCTTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA774320.4952422665405238No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT761810.4872410774269506No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA754890.4828151598677239No Hit
TCCTAGTACCGAAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750210.479821909264085No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA703260.44979346570834894No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA683260.4370017964620289No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA676870.43291485813782976No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA649050.4151216462161987No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC627790.40152410180736053No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT599410.38337272314683246No Hit
ACGTTGTGGCCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA596520.38152432694073923No Hit
TCCTAGTACCGAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA553930.3542844672807009No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA541580.3463856115210983No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG541040.34604023645144766No Hit
GTATCCTTCCTTGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537560.343814486002588No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530690.3394205476164771No Hit
CTCCCTAGTTCCTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA511920.32741556602880584No Hit
GTATCCTTCCTTGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA509880.3261108157656812No Hit
CAATCAGACCTATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA471910.3018258317015427No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG470450.3008920398465614No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA468630.29972799794514626No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC463730.29659403897979786No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC463030.2961463305561767No Hit
CATTGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTG459320.2937734759109843No Hit
CTCCCTAGTTCCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA452740.2895650167289451No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT444020.2839878489375496No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT435030.27823799361132867No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG429580.2747522637417065No Hit
TTCCGAGGATGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA425890.2723922007657605No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA421490.2695780335315701No Hit
CAATCAGACCTATGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA418670.267774408167839No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA416000.26606672032345524No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410300.2624210945882541No Hit
ATTCTTCAACCGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA405690.2594726148269773No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA402770.2576050311170146No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA388630.24856132095986638No Hit
ATTCTTCAACCGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA383270.24513315360185267No Hit
CATTGAACATGGTCTTGTGGATGAGCAGCAGGAAGTTCGGACCATCAGTG377340.2413404236703188No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359600.22999421304883294No Hit
CAATCAGACCTATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA351800.2250054620427682No Hit
ACAGTCACTGGGCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325010.20787102108732256No Hit
CAGTAGATGGAGCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320880.20522954138795751No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC312640.1999593736584737No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310680.1987057900723343No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281210.17985726543788189No Hit
CAGTAGATGGAGCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278510.17813039008962867No Hit
ACAGTCACTGGGCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273070.17465105605462963No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA269340.17226540974019094No Hit
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATT267180.1708839094615884No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA266720.17058970106892307No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT253880.16237744941278565No Hit
ACAGTCACTGGGCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251840.161072699149661No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA246830.15786838600345784No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG241200.15426753111061878No Hit
CCGTGTTCCTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236440.15122311382999462No Hit
ATTCTTCAACCGAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA233910.14960496767033515No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA233210.14915725924671394No Hit
TTCTTTGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229160.14656694622433417No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA228420.1460936544622203No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226780.1450447375840221No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216550.13850179876452942No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG216260.13831631956045778No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA214950.13747846522482382No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC213520.13656386087371195No Hit
CAGTAGATGGAGCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA202200.12932377608029486No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198140.12672706722329188No Hit
AGATGGGACTCACCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198010.1266439213731908No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC195050.12475075432473545No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG190830.12205171211376194No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT184410.11794558628569324No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG183950.11765137789302788No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175310.11212537677861764No Hit
CCGTGTTCCTCATTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160590.10271070821332616No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA159970.10231416646669024No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157930.1010094162035656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)