FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16338914
Sequences flagged as poor quality0
Sequence length91
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12328497.545477012731691No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11612697.107381800283666No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10244426.269951601434465No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3149271.9274659258259146No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2345801.4357135364076217No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1575620.9643358181578042No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1511200.9249084731090451No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1333760.81630884402721No Hit
TCCCTTGCGATTTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1314480.8045087941585347No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1086890.6652155706309489No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1028520.6294910420606902No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA999360.6116440786701001No Hit
TCCTAGTACCGAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA966560.591569305034594No Hit
TCCCTTGCGATTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA952630.5830436465973197No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA941380.5761582440546538No Hit
TCCCTTGCGATTTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA941150.5760174758248926No Hit
CTCCCTAGTTCCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA939370.5749280521336975No Hit
TTCTTTGCCTTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA897820.5494979654094514No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT879460.5382609884598205No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA871710.5335177111526507No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA828170.5068696732230796No Hit
TTCTTTGCCTTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA825570.5052783801909968No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT812220.4971077025070332No Hit
GTATCCTTCCTTGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA799690.4894388941639573No Hit
TCCTAGTACCGAAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA753170.4609669896053067No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA746260.45673782235465593No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710030.43456376598836377No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA707700.4331377226173049No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA699250.4279660202630358No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC677280.41451959414193623No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA649380.3974437958361247No Hit
ACGTTGTGGCCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA647800.39647677930124364No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT645070.39480592161755673No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA642610.3933003135948938No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA627990.38435235046833593No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG580840.355494863367296No Hit
TCCTAGTACCGAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA551350.3374459281687877No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA541080.3311603206920607No Hit
GTATCCTTCCTTGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA533910.3267720241382016No Hit
CTCCCTAGTTCCTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA533680.32663125590844044No Hit
GTATCCTTCCTTGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA523190.3202110005597679No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG512510.31367445841259706No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA506200.30981251263088844No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC505300.30926168042747515No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC500600.3063851122540947No Hit
CAATCAGACCTATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA477320.29213691925913804No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT474110.29017228440029735No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT473800.28998255330801054No Hit
CTCCCTAGTTCCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469500.2873507994472582No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG455690.2788985853037723No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA448040.2742165115747595No Hit
CTTTGTACGAGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA434440.26589282494540334No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431440.26405671760069244No Hit
CAATCAGACCTATGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426460.2610087794084723No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA420700.25748345330662736No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA406460.24876806377706623No Hit
TTCCGAGGATGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA405280.24804586155481326No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA401370.24565280164887335No Hit
ATTCTTCAACCGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391670.2397160545676414No Hit
ATTCTTCAACCGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA378710.23178407083849026No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA366780.2244824839643565No Hit
CAATCAGACCTATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA358630.21949439234455853No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC333800.20429754388816782No Hit
CAGTAGATGGAGCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331580.20293882445308176No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316930.1939725002530768No Hit
ACAGTCACTGGGCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA313900.19211803183491877No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA291900.17865324464037205No Hit
CAGTAGATGGAGCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA289930.17744753415067854No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA288900.17681713729566115No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT280060.17140674098657963No Hit
CCGTGTTCCTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA276130.16900144036500836No Hit
ACAGTCACTGGGCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA266290.16297900827435655No Hit
TTCTTTGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA266150.1628933232649367No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA263860.1614917613251407No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA261430.16000451437592486No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG252500.15453903484650203No Hit
CATTGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTG245020.14996100720035616No Hit
ACAGTCACTGGGCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA244370.14956318394233545No Hit
CTTTGTACGAGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238110.14573183994970534No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA237510.14536461848076318No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG234940.14379168652212748No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC230310.14095796085345697No Hit
CTTTGTACGAGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216590.13256082993031237No Hit
ATTCTTCAACCGAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA215390.13182638699242802No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA213240.13051051006205186No Hit
CAGTAGATGGAGCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211120.12921299420512283No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC207710.12712595218996806No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205860.12599368599406302No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA202750.12409025471337937No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT202250.12378423682259419No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG202110.12369855181317435No Hit
CATTGAACATGGTCTTGTGGATGAGCAGCAGGAAGTTCGGACCATCAGTG201600.1233864135645735No Hit
AGATGGGACTCACCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA194880.11927353311242106No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG194450.11901035772634583No Hit
CCGTGTTCCTCATTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183710.11243709343228074No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA177060.10836705548483822No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA176240.10786518614395058No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT169150.1035258524526171No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG167000.10220997552224097No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG165840.1015000140156194No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC165250.10113891290449292No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA164330.10057583998544825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)