FastQCFastQC Report
Fri 17 May 2024
EGAF00008204218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121442044
Total Bases115.6 Gbp
Sequences flagged as poor quality0
Sequence length1-1125006
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTACTTCGTTCAGTTACGTATTGCTCTACACGACGCTCTTCCGATCT4180610.34424733496745163Illumina Single End Sequencing Primer (95% over 24bp)
TTGTACTTCGTTCAGTTACGTATTGCTGGGCTACACGACGCTCTTCCGAT1986650.16358832036786206No Hit
ATTGTACTTCGTTCAGTTACGTATTGCTGGGCTACACGACGCTCTTCCGA1792480.1475996237349233No Hit
GATGTACTTCGTTCAGTTACGTATTGCTAAGCAGTGGTATCAACGCAGAG1405810.11575974462353417Clontech Universal Primer Mix Long (95% over 23bp)
ATTGTACTTCGTTCAGTTACGTATTGCTAAGCAGTGGTATCAACGCAGAG1388650.1143467249283123Clontech Universal Primer Mix Long (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)