FastQCFastQC Report
Sat 18 May 2024
EGAF00008204220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55509845
Total Bases57.5 Gbp
Sequences flagged as poor quality0
Sequence length1-1494353
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTACTTCGTTCAGTTACGTATTGCTAAGCAGTGGTATCAACGCAGAG1209760.2179361156566011Clontech Universal Primer Mix Long (95% over 23bp)
GTTGTACTTCGTTCAGTTACGTATTGCTCTACACGACGCTCTTCCGATCT1032710.1860408725695415Illumina Single End Sequencing Primer (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)