FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2730595
Sequences flagged as poor quality0
Sequence length28-91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATT183973867.37498603784157No Hit
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAACATT62375122.84304336600631No Hit
TGTGCAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATTG122490.4485835504716005No Hit
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACGGGTATTAAGATT80550.29499065222048676No Hit
NGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATT76250.27924316861343407No Hit
TGTGCAATAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATT76000.278327617240931No Hit
CGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCTGCCTCCTGAA63420.2322570721765769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60600.22192965269474235No Hit
TGTGCAAAAGCAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATTG48570.177873320649895No Hit
TGTGAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATTG48240.17666479283819095No Hit
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGAGACAGGGTC41360.15146881906690665No Hit
TGTGCAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAACATTG40310.1476235033023938No Hit
CGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCAGCCTCCTGAA39730.1454994241181867No Hit
CATTGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTG33830.12389241172711442No Hit
TGTGCAAAAGCAAGAAGTCCTGGGCAGCACAAGAAAGTCAATCACGTTGA33100.12121900171940547No Hit
TGTGCAAAAGCAAGAAGTCCTGGCACGCGAGACACACTGGTATTAAGATT28240.10342068303794594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)