FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704687
Sequences flagged as poor quality0
Sequence length28-91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATT181474767.09637751059549No Hit
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAACATT61360422.68669165785172No Hit
TGTGCAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATTG119540.44197350747055025No Hit
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACGGGTATTAAGATT80430.2973726719579752No Hit
TGTGCAATAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATT75920.28069791439822794No Hit
NGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATT73830.27297058772419874No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59090.2184725996020981No Hit
CGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCTGCCTCCTGAA58270.2154408255003259No Hit
TGTGAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATTG47990.1774327306634742No Hit
TGTGCAAAAGCAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATTG47300.17488160367539757No Hit
TGTGCAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAACATTG41480.15336340212379473No Hit
TGTGCAAAAGCAAGAAGTCCTGGCAAGCGAGACACACTGGAGACAGGGTC41280.1526239450258015No Hit
CGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCAGCCTCCTGAA35730.13210401055648952No Hit
TGTGCAAAAGCAAGAAGTCCTGGGCAGCACAAGAAAGTCAATCACGTTGA32690.12086426266699252No Hit
CATTGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTG32370.11968113131020336No Hit
TGTGCAAAAGCAAGAAGTCCTGGCACGCGAGACACACTGGTATTAAGATT29060.1074431163384155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)