FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500128
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGACATTGCCTCTTGGTCACCACT77800.1728839713003719No Hit
CTGTGAAAGCGTGCCTCCACCTGCAATC62660.13924048382623783No Hit
TCCAGAACAATTGAGACATTTCACAGGN62050.13788496682761023No Hit
ATGACCATCTTCACATCCTTACTTGGTN61520.13670722255011414No Hit
AGACAAAAGGACGCATTCAATCCAACCG59340.1318629158992811No Hit
TCCATCGGTTGGTGTTCACAACTGTGTN59330.13184069430913964No Hit
CATAGACAGTAATCCCATCTGCCACTTN58240.12941854098372313No Hit
CGGTCAGTCCCGAATACCTTTCCGCCAC49940.11097462116633128No Hit
ACTTCGCACAATTTTTTTCATTTCCTCN49700.11044130300293681No Hit
GCAACATTCGCAACATCAGTTACAACAC49030.10895245646346059No Hit
GACCTTCTCGTCCTCACTTATCTAATGC48930.10873024056204623No Hit
TAACTTCCACGTGAGACTAACTCAGCCN48390.10753027469440869No Hit
TAACCAGAGGTAGGCTACATTTCAGGCN48110.1069080701704485No Hit
TACATTCGTACACGTTAATATCTATTNN47570.10570810430281094No Hit
TCTCACGATTTTTTTCATTTCCTCATCN47370.10526367249998221No Hit
CAGCAGCATTTTTTTCATTTCCTCATCN46880.10417481458305186No Hit
CACACAAAGCACCTGCAGACTAATACGT46750.10388593391121319No Hit
CTGTACCGTCTAGATCATCACCGGCACC46630.10361927482951595No Hit
GGTAGAGAGGTCCGAAAACATACCACAN46490.10330817256753586No Hit
AGGACTTTCCACGGACGGGATATTCCGG46020.10226375783088837No Hit
AGGGTGAAGGTGCGATTGCTTTTAGCGC45880.10195265556890826No Hit
GAGACTTGTGGAGAAACTATAGACATGC45470.10104157037310937No Hit
TCCGATCTCTGTTGGACGCTTAATCGCN45250.1005526953899978No Hit
GTCCCATAGAAACCATCTTCCTGTACTT45230.10050825220971492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)