FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500128
Sequences flagged as poor quality0
Sequence length28-91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTG296838765.96227929516671No Hit
CATTGAACATGGTCTTGTGGATGAGCAGCAGGAAGTTCGGACCATCAGTG110197424.48761457451877No Hit
ATTGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTGC145290.3228574831649233No Hit
NATTGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTG121270.2694812236451941No Hit
CGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCTGCCTCCTGAA82220.18270591414288662No Hit
CTTGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTGC79470.1765949768539917No Hit
CATGAACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTGC75090.16686192037204275No Hit
CATTGAACAGGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTG57180.12706305242873092No Hit
CATTGACATGGTCTTGTGGATGAGCAGCAGAAAGTTCGGACCATCAGTGC53900.11977437086233991No Hit
ATTGAACATGGTCTTGTGGATGAGCAGCAGGAAGTTCGGACCATCAGTGC52950.1176633197989035No Hit
CGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCAGCCTCCTGAA51660.11479673467065826No Hit
CATTGAACATGGTCTTGTGGATGAGCAGCAGATATTGATCATAAACTTGA48160.10701917812115566No Hit
NATTGAACATGGTCTTGTGGATGAGCAGCAGGAAGTTCGGACCATCAGTG45530.10117489991395802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)