FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267478
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAATAGTGGTGACTGGCCTTCCTAT32171.2027157373690545No Hit
TGTAACGGTCACCACGGATGGCTGCAAG26500.9907356866732966No Hit
TGAGCATGTCTCGGACTTTATCTCCTAC25330.9469937714503622No Hit
TCAGCAACAAGTTTGCCCTACCTTCCAG24750.9253097451005317No Hit
TATTGCTGTACGCTATTTTATCACTAAC24610.9200756697747104No Hit
AGTGTTGTCTCCTGTGGTATCCATGTTA23690.8856803176335998No Hit
CACGAATGTTTCACTTACCCGTTTCACT22200.8299748016659314No Hit
ATCCTATTCTCACCCATCATAAAACAGA21930.8198805135375621No Hit
TCAGGGCAGCATTGAACTGAGCTTTAGC21650.8094123628859196No Hit
TGACTCCCAACTGCCGCCAACACTTTCA21360.7985703497110043No Hit
TTACAGGCAAAGGATTTTCTTGCGTCCA21190.7922146868153642No Hit
CTCAACCCAGAAGTTACATAACCATATT21170.7914669617688185No Hit
GGCTGTGCAACTGCTATCCTACAACCGT21060.787354474012816No Hit
GTTGTCCAGGTGATATTCAACCCTCAAA20700.77389542317499No Hit
AGTCACACAGTAGTTCACGCGTTACTCG20640.7716522480353525No Hit
GATCCCTCATGGATCTCCAACATTGTCC20250.7570716096277077No Hit
ATTCACTAGGTAGGCTAATCGTTGTAGA20230.7563238845811618No Hit
ATACTTCGTCTCTCACGCACCGAATTTC20130.7525852593484323No Hit
TCGACGGCAAATCAGAATTACATCACCT19730.7376307584175147No Hit
CAATACGTCGCTCTCAATTTAATCCTGG19550.7309012329986018No Hit
TTGTGGATCAAGCTGTTAAATTCTCCAG19000.7103387942185899No Hit
ATTTCACAGAATCGCGATTTTATCCACC18460.690150217961851No Hit
CATTGTTCAAAGACTATTTTTCGTTACC18400.6879070428222134No Hit
GACATCAGTTGGGTAGGACTTGGTTCCT18110.6770650296472981No Hit
TCTCACGGTTCTCAGAAGTCTCCATATG17700.6617366661931074No Hit
TCACTATTCAAGCTGTCATATGTCGCCT17590.6576241784371051No Hit
GGGAAGTGTAACGCGAACCCGTCCTGTC17350.6486514778785545No Hit
TCAAGACAGGCGACATGTATTAAAGAAC17220.6437912650760063No Hit
CAAAGAATCCATTCATAATTATTTGGCA17050.6374356021803662No Hit
CGGTCAGCATCGCCTTTCAAATCCATTT17040.6370617396570932No Hit
ACGTACATCCGATGCGGCCGGTGCACCA16910.632201526854545No Hit
CCACCATGTGATATAGATTAAACGAGTT16700.6243504138658132No Hit
AGACACTGTGGGCTCTTTGCATTGTTAT16160.6041618376090744No Hit
ATATCCTAGGTTGGTGGTTTCTGTAATT15850.5925720993876132No Hit
AACCTGAGTCTTACAGCCAAAGGATCTT15820.5914505118177944No Hit
TGGATGTAGTGACCTTGCGTATTTTCTT15820.5914505118177944No Hit
TCCCAGTCACTGGCCAATCCATGTCCAA15720.587711886585065No Hit
CGCGTGATCGGCCCAATTTAACATATAT15650.5850948489221544No Hit
AGCGCCACACGCAGTCGGTTCTAGTTAA15540.580982361166152No Hit
TAGATCGCACTTGACACTATGACCTTTC15430.5768698734101496No Hit
TAGGGTTCAGGTGTTTTGAACTCCAACA15400.5757482858403308No Hit
CGTTAGAAGGCTAACGACACCGGTACAC15310.5723835231308743No Hit
TCGAAGTCATACATCGCTTTTTTCTTCA15080.5637846850955966No Hit
GGTTGTACATGAAAGTAACGTACCCTTT15060.5630369600490508No Hit
TTTCATGTCAAGTTGCTCCTTCGCCTTG15010.561167647432686No Hit
TACTGCCTCGTCCTCATACTGCCGCCCG14990.5604199223861401No Hit
AGATGCTCAGCTATACCACTCGTCTTCT14860.5555597095835919No Hit
CAGATTGGTAGCTGCCACCGTTCTCCAT14770.5521949468741355No Hit
TCCTGCAAGGCCGCTTGATTTCCGTTAA14720.5503256342577707No Hit
AGTCACACAGTAGTTCCCAATTGTCGGC14660.5480824591181331No Hit
TTCCGGTCATCCAACACGCTAACTTCCC14650.5477085965948602No Hit
AGTTCCCCACCCTCTAGGCGTTAAATAT14520.5428483837923118No Hit
TCTGTCGGTCTCGACGACTAGGTCCGTA14500.542100658745766No Hit
GTGAGCCGTCAATCTGCTCTCTTTATGT14260.5331279581872154No Hit
AGATGCTCAGCTATACTCATTATTCTTA14160.529389332954486No Hit
CAATACGTCGCTCTCAACAACTTTTATA14140.52864160790794No Hit
TGTTCCGCAAATTGCCAGGCAGGTTCGT14050.5252768451984836No Hit
TAGGTTGTCAAGCCTACCGTCTTCTGCC13990.523033670058846No Hit
CGAGTTACAAGTCATCATAGCCTCTAAC13950.5215382199657541No Hit
TCTAACTGTATCTTCTACAATTCCCTAC13940.5211643574424812No Hit
GAATCGTTCAGCTCTCCAGCTCTCCAGA13740.5136871069770224No Hit
GTCATGAGTCAACACTTAGTCAACATCC13480.5039666813719259No Hit
GTCAGCGCACGAGAACCGCTCGAATTGT13460.50321895632538No Hit
ACCACAATCCACATAGCCCTTACAACCC13320.49798488099955884No Hit
TGAGGAGTCGCTACAAGTCCACCGGTTG13280.4964894309064671No Hit
TGGGAGAGTTGTTGACAGATGTGCATTG13180.4927508056737377No Hit
AGAAGTACATTATGCGCTTACTATGTTC13110.49013376801082703No Hit
TTCCGTGGTTACGTACCAGTTCTTTCTA13010.48639514277809764No Hit
TTTGGAGTCTGATGGTTTCCTATTGCTT12980.4852735552082788No Hit
GCTTGGGAGGTTCACTGGGTTACCTGCA12950.48415196763846No Hit
CATTGTTCAAAGACTAACAAAGAATCCA12880.48153492997554936No Hit
AACCATGTCTACTGCCTTCACCTACCCG12790.4781701672660929No Hit
CCCGGAATCCTCTGCATTCCCTAGCCCC12780.47779630474282No Hit
GTGGTTAGTCTTGCTCGATCCTGTCATT12740.4763008546497282No Hit
TGTAACGTCAATCTCTAGTTTTTTTCAC12580.4703190542773611No Hit
GTTCTATTCCATTCGCTTTCTTGTATCT12470.4662065665213587No Hit
ACACAGTAGGCAATGCACTTCGTAGGAC12450.46545884147481287No Hit
CTCAACCCACTTCCTGTCTCCAACTGTG12390.4632156663351752No Hit
CCGCAAGTCCGATGTACACATTCATGTT12350.4617202162420835No Hit
TCATTTGTCCGACGGTAAATAGGGTTCT12210.4564861409162623No Hit
TTCTTGACAGAGAGGGATAACCATTCGT12200.4561122783929893No Hit
ATCTTCAGTACAGAGCCATATTCGTCCA12190.4557384158697164No Hit
TGCAGATTCTATCGTTCCCATCTATCCA12110.45274751568353283No Hit
CATTGAGGTAAGTTAGATATTTTCTAGA12090.4519997906369869No Hit
TCATTTGTCCGACGGTATCCTCATCCAT12070.45125206559044106No Hit
CATCGGGAGCTGTTACGGCCCGCATGGT11790.44078391493879876No Hit
AGCCAATAGTGCCTCGTGAATCAACCGG11760.43966232736897987No Hit
TCCTCCCCATTCTCTACCGCACAGTTTC11700.43741915222934225No Hit
GGATGTTCAACCACGCTAAGGTCTCCCT11700.43741915222934225No Hit
CTTCCTTTCCTTATCAGAAATTGTAACT11650.4355498396129775No Hit
GATAGAACACCCTTGTCACATTGTCTTT11490.4295680392406105No Hit
GTTAGACCAAGCAATACATGCTACTAAG11380.42545555148460806No Hit
CATGCCTTCTCACCCAATACATTTAGCA11240.4202214761587869No Hit
ATCACTTAGCAAATGTCCAATTCAAATT11160.4172305759726033No Hit
AGCGCCACACGCAGTCCACCAACCTCCT11140.41648285092605747No Hit
CCGGTGACAGACCATTTGTCCCGTACAC11100.4149874008329657No Hit
CATTGAGTCATTCCTAGTGTGTCTCTCC11090.4146135383096928No Hit
GGTGTCGTCTCGGTCTGTCCTCCTTGGG11040.412744225693328No Hit
GGATGTTCAGCCTATCACCAGACCCCGT11000.4112487756002363No Hit
GTACAGTCAAGAGCTGAATTTCATCACA10930.40863173793732566No Hit
GATTCGACAGCCTTCTCTAGCTTTATCC10890.4071362878442339No Hit
CAGAGCCAGGCATGGTCATCATCTATAC10850.4056408377511421No Hit
TAGACCAGTTGCATCAGCTCTAGGTTTG10850.4056408377511421No Hit
GTGCAGCGTCTGTCAAAATGCTCCACTT10780.4030238000882315No Hit
ATGAGTCGTAACATCCTTTGTCTTCTTA10740.40152834999513976No Hit
GTTTGGAGTCTCAGATACTCCATGAATT10590.3959204121460456No Hit
TATTCCATCCACATAGCTTCGGTCGGCC10350.386947711587495No Hit
TCATTTGTCCGACGGTTATTGTAAACCT10270.3839568114013115No Hit
GCACGTGTCCGTGTCTCCATGAAGTGAT10190.38096591121512796No Hit
CTTCCTTCACGCAGTCTCCGTCAATTCA10100.3776011485056715No Hit
TCTGTCGGTCTCGACGCTCTTCTGCACC10070.37647956093585266No Hit
CTCTGGTCATAACCCATAAAAGGCCATT10070.37647956093585266No Hit
GGCGTCACATTGTGCACACTGAAAGCCT9880.36937617299366676No Hit
GGGACTCAGAACCGCACTCTCCATGGCT9860.3686284479471209No Hit
GTCATGAGTCAACACTAAGATTCGCACA9850.368254585423848No Hit
TTACGCCTCGATGGAGCAATAACCAAAA9840.367880722900575No Hit
AGGTTGTAGTGATTCCGCCTTTTCTTAA9800.36638527280748323No Hit
CTCAATTGTTCTGACATCGCCATCAAAA9760.36488982271439147No Hit
CATCCGTTCTCTTGCAGATACATATTCA9680.36189892252820793No Hit
CCATAAGCAGACCATTCCATCGTAACAT9670.361525060004935No Hit
TCAGGGCAGCATTGAAACAACACCAGCT9600.3589080223420244No Hit
TCCGATCAGCTAGCCCGCGCCCTCCTGA9550.3570387097256597No Hit
GACTATGAGACGATATATCATTAGAATT9420.35217849692311143No Hit
TTCCACGTCTTCACATCGCATCTAAGGT9230.3450751089809255No Hit
GGGTTTAGTACGGGATGTTTATTAATGA9200.3439535214111067No Hit
AGGTTGTAGTGATTCCCGCTTATTTTCC9170.3428319338412879No Hit
TCGAACAGTCATAGTCAAACCTAGGCAG9170.3428319338412879No Hit
CTGCGAGTCGTAGCTATTCTGTTCGTCA9150.342084208794742No Hit
CAATACGTCGCTCTCAATTACGCCGGCT9140.34171034627146907No Hit
AGCGATTAGAAGATCTATCCTCTGCCAT9110.34058875870165023No Hit
TCATATCAGCCTGCCACCGACTCTCATT9090.3398410336551043No Hit
GGTCACGCATCCGCGAACCGAAATACCA9000.33647627094564786No Hit
GGGATTCAACATACCGACCATTCTATAT9000.33647627094564786No Hit
CGCAGGTTCTAGGCCGCTGTCTCCCCAT8950.33460695832928317No Hit
CAGGCCATCCACCTCATTGATCTACATG8930.3338592332827373No Hit
TAGGTTGAGATGAAGGCCCACGTCACTA8880.33198992066637256No Hit
GATCATGGTAGCTAAATTGACCAACTGA8780.3282512954336432No Hit
TTGGGTAAGACATAACTCTATCACACAT8630.322643357584549No Hit
GAAGTAAGTATAATGGGTCTGTTGCAAC8600.3215217700147302No Hit
ACATCCCTCCAGTACACTTCTTCAGGTT8560.32002631992163844No Hit
GTTATGGCACACACGCCATCCCTTAGTA8550.3196524573983655No Hit
GTATTGGTCCGTCACTGCTTTTTAGTCA8520.3185308698285466No Hit
AGGATAAGTCGAACGAGTCTACATCACT8480.31703541973545485No Hit
GAATCGTTCAGCTCTCCGAATGCCTGCC8460.316287694688909No Hit
ATCATTCAGTGGCAGTGTAAAACGGTCG8450.31591383216563607No Hit
CTAGGTACACTGTGTAATATTTTGTCGG8400.3140445195492714No Hit
TTGTGTTAGCTTTCTTGTCTTAGTTCAA8350.3121752069329066No Hit
CAGATTGTCGAAGTGGAGGTAGCGGCTG8310.31067975683981486No Hit
CTCATTAGTCAGTCCGCACCAGCCTACC8280.309558169269996No Hit
CATTGAGTCATTCCTAAGTGCCCTGGGC8250.30843658170017724No Hit
ACTGTGACACCCGTAGATAGGTGGCCCA8120.30357636889762896No Hit
TATCTGTCACCTGAATCCCTACTGTATG8070.30170705628126426No Hit
GTCTTTATCGGTGCACAACGCTTTATAA8040.3005854687114454No Hit
AGCCAATTCGAACCATGACGATACGGTC7880.29460366833907836No Hit
GGGTATTAGTTGGCGACTTTAGTACATA7870.29422980581580543No Hit
TGTAGACTCAACCCGGGCACGTTCATCA7860.2938559432925325No Hit
TAGATCGTCACCTACCTCACAGCCATTT7760.29011731805980306No Hit
ATACCTTAGGCTAAATCCCGCTACCCTG7730.2889957304899842No Hit
GAACGTTAGTTGGACGTTACAATCTCCA7700.2878741429201654No Hit
TAAGTCGGTGGCTGCTTACCTTTCGAGA7510.2807707549779795No Hit
TCATGCCTCCATAGACGCTTTATCTGGT7500.28039689245470656No Hit
GATCACAGTAGACTGGTCTTTTGAGCGC7490.28002302993143363No Hit
GCACGTGTCCGTGTCTCCAAGTAATATC7460.2789014423616148No Hit
AGGAATACAAACCATCCACCATTTTCAC7400.2766582672219771No Hit
GTCCCATTCGGTTGTATCCCCCCTTATT7380.27591054217543126No Hit
CAGGCCATCCACCTCACCATACTTTAGC7210.2695548792797912No Hit
GAGTCTACATCGCTCTCAATATCAGCCG6940.25946059115142184No Hit
CCATAAGGTCCGGCATTACTCTATGACA6890.2575912785350571No Hit
CATTCTAAGTCAGGGTCATTGTCTATTC6850.2560958284419653No Hit
CCGATCTGTCCAACGCACACCCAGCACC6810.25460037834887356No Hit
CATCCACGTAGTGGCATCTTTTGACATT6790.25385265330232765No Hit
CTAACCCTCTCCGATCAGACTTTTAGTG6770.2531049282557818No Hit
TAGATCGTCACCTACCGATTACCTACAG6680.24974016554632528No Hit
GGTTGTACATGAAAGTTTAAACCATTTC6660.24899244049977942No Hit
TGATGCATCATTGCTTAATGTGCCCTAT6620.24749699040668766No Hit
TCACAAGCATTCCTATTGGTTCATTGCC6490.2426367776041394No Hit
CTCTGGTCATAACCCAGTCTAGTCCCTA6470.24188905255759352No Hit
GCCGTGAGTCACTCGGGCTCACCTTTGA6450.24114132751104764No Hit
GATAGCTCAAGGACACCGCAATGCTGCC6310.23590725218522643No Hit
TCGTCCACATTGAAAGACAATCATCTGT6200.2317947644292241No Hit
AGACCATCACCCATAACACCATTGAGGT6060.2265606891034029No Hit
CTCAGTCGTTCGTGCGCAATTGGCTGTC5760.2153448134052146No Hit
GTCCTCAAGAGTCAATTTATTTCATGAT5680.2123539132190311No Hit
ATCGGATAGTGCGTCCCTTTGTCTTTAT5660.21160618817248522No Hit
AAGCGTTCATCTGTTTGTGCTATTCCTG5660.21160618817248522No Hit
GGTGATTGTCACTTCCCATCTCAATGCT5630.2104846006026664No Hit
ACACAGTAGGCAATGCACTTCATAGGAC5200.1944085121019299No Hit
ATTCAGGGTGACTCTAACTTGCTTCTCT5110.1910437493924734No Hit
AATGGAAGTTACGGAGGGCAATCTAGGA5090.19029602434592752No Hit
ATCCGTCCAATAACCCGCGCCCTTTTTG4840.18094946126410397No Hit
ATTCGTTCAAAGTGTATTCTAAGTTGCC4760.17795856107792044No Hit
CAAGAGGGTCGGAACACATAATTTTTCC4650.17384607332191807No Hit
GATTCGACAGCCTTCTTATTTCCTATAA4590.17160289818228042No Hit
TCATTGTGTTACCTTTCTCTGGCCAACC4490.167864272949551No Hit
AGTGACTCAAGCGGATGCGGAGTCAATT4470.16711654790300512No Hit
GTTGCTCCCAAATTGCCCATAATTTTCT4230.1581438473444545No Hit
ACTGTCCTCTCAACCCTCCCAAATGGCC4180.15627453472808978No Hit
GCACGTGTCCGTGTCTACAATTTTGCAT3900.1458063840764474No Hit
TGGTGATCACAGAGACGTGGAATATCCG3890.14543252155317446No Hit
CCCTAACTGTACTGTTCGGCTTCATCTT3740.13982458370408035No Hit
CCCAACTAGCGACATGACCGGAATCTGC3690.13795527108771563No Hit
GATTCTTTAAGATGGGGGTGAGTCTAGG3470.12973029557571092No Hit
CCACCATTTTTCTGTACTGTTCGGCTTC3460.12935643305243796No Hit
GACATCACAGGAACCAGTCCTACCATCC3440.12860870800589205No Hit
ACGGGTCTCCCGTGTTACTACATGTCAG3370.12599167034298148No Hit
ACCAAACGTGACAGGTTATTCTTTATCA3340.12487008277316264No Hit
CATCCACGTAGTGGCAAATCCATAAAGT3300.12337463268007089No Hit
TCCACCATTTTTCTGTACTGTTCGGCTT3160.11814055735424969No Hit
TACATTCTTCTGTACTGTTCGGCTTCAT2900.10842013174915321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)