FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291117
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTTCATTGCTTTAACGTCACCACC5930.2036981694645108No Hit
TGACTCCCAGCAGTCCTACTCTTGCATA5730.19682807943198094No Hit
ATCCCTGGTAAGATTGGTGAATCATCAC5170.1775918273408973No Hit
GCTTGGGTCAAGAGGCACATCGGAATTT5060.17381327782300587No Hit
ACCCTCATCACGATCAGTCGGTCACATT4880.16763019679372898No Hit
CTATAGGCAATTCTCTAACTTGCTGGTC4800.16488216078071702No Hit
CTTGATTTCGTGGACCATCAAACTCTCT4670.1604166022595726No Hit
CATACAGAGACTTCCACGCAGGCCGTCC4630.15904258425306664No Hit
TATTGGGTCACATTGGTCTAGCCGTTAC4590.15766856624656067No Hit
TACAGGTCATATCTCTAGCTCCACCTGA4490.15423352123029574No Hit
TTCCTCTTGACAAGTCTTCATGACAACA4470.15354651222704271No Hit
GTCATTTAGTCAGCGACATAGCCTCTCT4400.1511419807156573No Hit
AAGAACATCACCCTGTCTACTTACCCCC4390.15079847621403078No Hit
CGGACACCACAAGTTCTTCAAAGCTCCA4380.1504549717124043No Hit
CATACAGTACAACACCCAAGTGTCCCAT4320.14839394470264533No Hit
CCTTCAGCAACACTACATATGGCTTCTG4190.14392838618150092No Hit
GCAGTTAAGTGTTCCATCAATTTTTCCT4150.14255436817499492No Hit
CAACGATAGTCTGGTTCTTAGGTTAATG4050.13911932315873No Hit
GTATTGGCATATCTCTTGTTTTGTATTT4010.13774530515222402No Hit
TCACAAGCACTGGCCAGGTATCTTCAGC3990.13705829614897103No Hit
CTATCCGAGCTTACGTGCCGTCACATCT3970.13637128714571806No Hit
TGGATCAGTTATGTCGAATCGGCTACAT3940.13534077364083857No Hit
GTAGATCAGTACAGATCCAACTCTATAA3930.13499726913921206No Hit
AATTTCCTCCTCTTTCCCAACGCAGTAG3900.13396675563433258No Hit
AGAGAGCCAGACGCTCTCTGTTATTGAG3800.13053171061806765No Hit
CCCTCAAGTCCAGCCAGAGCTGTAAGCC3790.13018820611644116No Hit
TACTTGTTCCGGCAGTAATTAGGCTTCT3790.13018820611644116No Hit
CCCTCAAGTCCTGTCTTCCTGTTCGGCA3770.12950119711318817No Hit
TGGGTTATCTCGTTTACAAGGCAGTACC3740.12847068360830868No Hit
GGAGGATCATTCATCTTACTTATAAACA3730.1281271791066822No Hit
TGTTCCGAGGTTGTTCGGTCCTGCCATT3710.1274401701034292No Hit
CATGCTCCAATCCTTTTACAGATGAACT3680.12640965659854975No Hit
TAACTTCCAGACCAAGGTCATCATCACT3670.12606615209692323No Hit
TGGGTTACAAGCCTGCGAAATCGCTCCT3670.12606615209692323No Hit
AGCCAATCAGATTAAGAGTATTTTAGCT3650.12537914309367026No Hit
CAGTTCCGTCTACAACCATTTTGACCAG3650.12537914309367026No Hit
TACGTCCGTACGGATGTTAGTAGTCATG3640.12503563859204375No Hit
ATTTCTGAGTACAACATCTACTTTTCTC3620.12434862958879075No Hit
CTGCATCTCGTGCGACTTTCTTATGTCA3590.12331811608391127No Hit
CGGGTGTCATTCGATGGAGTACTTAGTA3590.12331811608391127No Hit
CAGCAATCACCAGGTCCACTCAGAAAAC3570.12263110708065829No Hit
TTGCCTGCAATACGAATCCGCAACCCTT3540.12160059357577882No Hit
CAGCAGCAGTGCTACTGTCTACTCAGAA3540.12160059357577882No Hit
CATCGTCTCGAGTCCGCCGCTTAAGTCC3540.12160059357577882No Hit
GAGCCTGTCATTCCTAACAATTACGGCC3520.12091358457252581No Hit
CGGGTGTCATTCGATGAGCCAAACATTG3500.12022657556927284No Hit
ACGCACGGTTCGGCCATTCCAACGCTCA3480.11953956656601986No Hit
TTTGTTGCATCTATCTCTGTCAGCTAGG3460.11885255756276686No Hit
TGCAGGCGTGCAACAGTTGCTTTGGCCT3430.11782204405788738No Hit
GTGCAGCTCCATATGGTAGCGAGGTAAT3430.11782204405788738No Hit
ATGCCTCTCCGTTTCGACGAGTCCCGGT3430.11782204405788738No Hit
TCAGCCTCACCTAAACAAAGATTTGTAC3420.11747853955626089No Hit
CGGACACTCAAGGCTTTATGTAGTATAT3380.11610452154975491No Hit
ATAGGCTAGACATCCTGCGAGTAACCAT3380.11610452154975491No Hit
CATACAGAGACTTCCACTACAACTTTTT3370.11576101704812841No Hit
TCCCAGTTCGAGCACCTTAAGCCGTCAA3370.11576101704812841No Hit
TGCAGTACAAGCCATTTCTTATAGAAAG3370.11576101704812841No Hit
GGCACGTAGCTAATGAGTGCCATCAAGA3330.11438699904162243No Hit
TTTCAGTAGAATCTAGACACTCATATTT3330.11438699904162243No Hit
TGTAGACAGTCCGCGTCTATCTCTACTA3290.11301298103511648No Hit
TTTAGTCTCGTCACCTTACGTCCTAATC3290.11301298103511648No Hit
CGGGTGTCATTCGATGTCTTTGCTCCAG3290.11301298103511648No Hit
TGGGTTATCTCGTTTAGTTCTTGACCTT3280.11266947653348998No Hit
AAGACTCAGGCGTCCTAGGGGTTGCACT3280.11266947653348998No Hit
GACTGATTCACTTCTACGCGGCCCTCTG3280.11266947653348998No Hit
AAACGCTAGTCAGGGTCTATAGGCTATT3270.11232597203186348No Hit
AGCCAATGTTGCTGATTTGCCATCACCC3270.11232597203186348No Hit
AATGCCATCAACACGTTTACTAATGGTC3260.11198246753023697No Hit
CGTAATGGTATACCTGATTTCTCCATAC3250.1116389630286105No Hit
GGAATCTTCCACAGCGATCCTTGTCTCT3240.111295458526984No Hit
CTCCGATTCGCTATTTTTGTACCCCTCG3230.1109519540253575No Hit
TAAGCACCAGTTAGGGAACTACTATAGC3230.1109519540253575No Hit
GTTTGGAGTGACACGATCTTATCAAGAT3180.10923443151722503No Hit
TGCCGAGGTCCTACAACGACCTCAGTAG3150.10820391801234555No Hit
CCTTCAGGTTGCATACTTCTCGTTAATT3140.10786041351071905No Hit
GAAGTAAGTGCGAGTAAAAGACATCGGG3120.10717340450746608No Hit
GCATCTCTCTGGGCCAACATCAGCCGGG3100.10648639550421307No Hit
ATTCACTCAAAGGTTACCTCTAAAGCCA3090.1061428910025866No Hit
CTTCTCTGTGATAGATTCTTTGGGCCTT3090.1061428910025866No Hit
TATTGCTCAACTGCTATACTACCTTTCT3080.1057993865009601No Hit
GTTGAACTCCCACAGGTGTATCCCAGCA3070.10545588199933359No Hit
TTGAGTGGTGATACAAGACTTAACACAT3070.10545588199933359No Hit
GTTTACTGTGATCATCGCCTCCTTCTAC3070.10545588199933359No Hit
TCAGGGCCATCACAGTTAAGTCCTCTCA3060.10511237749770712No Hit
GACCCAGGTAGGACCACTTTTTTACTTC3050.10476887299608062No Hit
AATGGAAAGCTGCCACCGTCATCAACTC3050.10476887299608062No Hit
AGGAGGTGTCTTCCGTTATTAGTCGCCC3040.10442536849445412No Hit
GGGACAATCAAGTTGCTCTTACAGGTTC3040.10442536849445412No Hit
TTCACGCCAGAAACCGATACAATATCCA3040.10442536849445412No Hit
CTCCCTCAGCCTCAGCCTATCCCTCATC3030.10408186399282762No Hit
GAACGTTGTTTCAGACCGATGTATGGCC3030.10408186399282762No Hit
GCCATTCTCAAGCGTTCAATCAATATGT3000.10305135048794814No Hit
AGGTGTTCATCAGCATTATATATTTCAC3000.10305135048794814No Hit
CTCCGATGTAGCTGTTTCTGCCAATCCA3000.10305135048794814No Hit
GATTCTTCACTTCTCGGTTCCCGTCTGA3000.10305135048794814No Hit
TAGGGTTGTACTCGTACGACAATGGTCG2990.10270784598632166No Hit
AGGTCATGTTCAGCGCCTTCACTCCTTA2980.10236434148469516No Hit
CATACTTAGTCAGCCCTTTATCTCAGAT2970.10202083698306866No Hit
ACAGCCGAGGCTATCTGAACAGCAAACC2960.10167733248144217No Hit
GCTTTCGTCGTCTAAGCTCCGAACCTTT2950.10133382797981567No Hit
GGGTCACCATGACGTTACGGCCGACCAG2950.10133382797981567No Hit
TCGGGCAGTTCTCTCGGGGGCAGTCACC2940.10099032347818918No Hit
AGCCAATAGAGCAGCTTAAACCCATCTA2940.10099032347818918No Hit
ATCGCCTCAGTAACAACCTACGGGTCCC2940.10099032347818918No Hit
CTGAGCGCAGTTAGAATGTCCAGACCTG2940.10099032347818918No Hit
CCCTCAATCCAGCTCTCACGTTTTAATG2930.1006468189765627No Hit
CGAGAAGTCATTGGTGACCTAAAAATTG2930.1006468189765627No Hit
CCACAAATCCGACAGCCTATAAAAAGAC2920.10030331447493619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)