FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008204259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288381
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTTCATTGCTTTAACGTCACCACC6000.2080580898186774No Hit
TGACTCCCAGCAGTCCTACTCTTGCATA5530.1917602061162143No Hit
CGGACACCACAAGTTCTTCAAAGCTCCA5210.18066377465921818No Hit
ATCCCTGGTAAGATTGGTGAATCATCAC5100.17684937634587578No Hit
GCTTGGGTCAAGAGGCACATCGGAATTT4970.17234145106647109No Hit
CTATAGGCAATTCTCTAACTTGCTGGTC4910.1702608701682843No Hit
TATTGGGTCACATTGGTCTAGCCGTTAC4900.1699141066852532No Hit
CTTGATTTCGTGGACCATCAAACTCTCT4740.16436589095675513No Hit
CATACAGAGACTTCCACGCAGGCCGTCC4720.16367236399069288No Hit
CATACAGTACAACACCCAAGTGTCCCAT4680.16228531005856836No Hit
GTCATTTAGTCAGCGACATAGCCTCTCT4560.15812414826219479No Hit
CCTTCAGCAACACTACATATGGCTTCTG4520.1567370943300703No Hit
GCAGTTAAGTGTTCCATCAATTTTTCCT4440.15396298646582124No Hit
TACAGGTCATATCTCTAGCTCCACCTGA4370.15153564208460335No Hit
ACCCTCATCACGATCAGTCGGTCACATT4320.14980182466944772No Hit
TTCCTCTTGACAAGTCTTCATGACAACA4310.14945506118641658No Hit
AAGAACATCACCCTGTCTACTTACCCCC4310.14945506118641658No Hit
GTATTGGCATATCTCTTGTTTTGTATTT4140.14356008197488737No Hit
CTATCCGAGCTTACGTGCCGTCACATCT4110.142519791525794No Hit
TAACTTCCAGACCAAGGTCATCATCACT4000.1387053932124516No Hit
AATTTCCTCCTCTTTCCCAACGCAGTAG3930.13627804883123368No Hit
GTAGATCAGTACAGATCCAACTCTATAA3900.1352377583821403No Hit
CGGGTGTCATTCGATGAGCCAAACATTG3890.13489099489910916No Hit
TGCAGTACAAGCCATTTCTTATAGAAAG3860.13385070445001576No Hit
CGGACACTCAAGGCTTTATGTAGTATAT3750.13003630613667336No Hit
CGGGTGTCATTCGATGGAGTACTTAGTA3750.13003630613667336No Hit
ATGCCTCTCCGTTTCGACGAGTCCCGGT3740.12968954265364221No Hit
TGGATCAGTTATGTCGAATCGGCTACAT3730.1293427791706111No Hit
AGCCAATCAGATTAAGAGTATTTTAGCT3720.12899601568757998No Hit
TTGCCTGCAATACGAATCCGCAACCCTT3710.12864925220454884No Hit
CCCTCAAGTCCTGTCTTCCTGTTCGGCA3690.12795572523848658No Hit
CATGCTCCAATCCTTTTACAGATGAACT3680.12760896175545547No Hit
TGGGTTACAAGCCTGCGAAATCGCTCCT3680.12760896175545547No Hit
CTGCATCTCGTGCGACTTTCTTATGTCA3640.12622190782333093No Hit
ATTTCTGAGTACAACATCTACTTTTCTC3590.1244880904081753No Hit
CTTCTCTGTGATAGATTCTTTGGGCCTT3590.1244880904081753No Hit
GGAGGATCATTCATCTTACTTATAAACA3560.12344779995908191No Hit
ATAGGCTAGACATCCTGCGAGTAACCAT3560.12344779995908191No Hit
CAACGATAGTCTGGTTCTTAGGTTAATG3550.12310103647605079No Hit
CAGTTCCGTCTACAACCATTTTGACCAG3540.12275427299301965No Hit
ACGCACGGTTCGGCCATTCCAACGCTCA3500.12136721906089515No Hit
CCCTCAAGTCCAGCCAGAGCTGTAAGCC3440.11928663816270836No Hit
CTCAGGGCAATACCCATCAAATCTCCCC3430.11893987467967723No Hit
GTGCAGCTCCATATGGTAGCGAGGTAAT3430.11893987467967723No Hit
GGCACGTAGCTAATGAGTGCCATCAAGA3420.11859311119664609No Hit
TCCCAGTTCGAGCACCTTAAGCCGTCAA3410.11824634771361497No Hit
AAGACTCAGGCGTCCTAGGGGTTGCACT3400.11789958423058386No Hit
CAGCAGCAGTGCTACTGTCTACTCAGAA3380.11720605726452159No Hit
TACGTCCGTACGGATGTTAGTAGTCATG3350.11616576681542821No Hit
AGAGAGCCAGACGCTCTCTGTTATTGAG3340.11581900333239707No Hit
TGTTCCGAGGTTGTTCGGTCCTGCCATT3300.11443194940027257No Hit
GAGCCTGTCATTCCTAACAATTACGGCC3270.11339165895117916No Hit
TGCGATAAGGTGTGACCATTTGAGTACA3270.11339165895117916No Hit
TTTGTTGCATCTATCTCTGTCAGCTAGG3260.11304489546814804No Hit
TGCTTGCGTTGCATTGGGTAATAATTTA3250.11269813198511693No Hit
AATGGAAAGCTGCCACCGTCATCAACTC3250.11269813198511693No Hit
TTGCTGCCATCTTAGGGTATTCCGGTTT3240.11235136850208578No Hit
TGGGTTATCTCGTTTACAAGGCAGTACC3240.11235136850208578No Hit
GACTGATTCACTTCTACGCGGCCCTCTG3240.11235136850208578No Hit
AGCCAATGTTGCTGATTTGCCATCACCC3240.11235136850208578No Hit
TACTTGTTCCGGCAGTAATTAGGCTTCT3240.11235136850208578No Hit
CTCCCTCAGCCTCAGCCTATCCCTCATC3230.11200460501905465No Hit
CATACAGAGACTTCCACTACAACTTTTT3230.11200460501905465No Hit
TGTAGACAGTCCGCGTCTATCTCTACTA3180.11027078760389901No Hit
TCAGCCTCACCTAAACAAAGATTTGTAC3170.10992402412086787No Hit
TAAGCACCAGTTAGGGAACTACTATAGC3170.10992402412086787No Hit
GTTTACTGTGATCATCGCCTCCTTCTAC3170.10992402412086787No Hit
AGCGTATCAAACACCTAAACCCAGATTA3160.10957726063783675No Hit
TCACAAGCACTGGCCAGGTATCTTCAGC3150.10923049715480564No Hit
CCTTCAGGTTGCATACTTCTCGTTAATT3150.10923049715480564No Hit
TTTCAGTAGAATCTAGACACTCATATTT3140.1088837336717745No Hit
CCTTGTGGTTAAAGTGAACTGTCTCCGG3140.1088837336717745No Hit
ATCGCCTCAGTAACAACCTACGGGTCCC3140.1088837336717745No Hit
CTCAGAAGTAGTCACTCTTATCGATGGG3120.10819020670571222No Hit
CAGCAATCACCAGGTCCACTCAGAAAAC3110.1078434432226811No Hit
TCAGGTAGTACTGAGGCTTATTCTTACC3110.1078434432226811No Hit
CGGGTGTCATTCGATGTCTTTGCTCCAG3100.10749667973964999No Hit
TTCACGCCAGAAACCGATACAATATCCA3080.10680315277358772No Hit
ATTCACTCAAAGGTTACCTCTAAAGCCA3070.10645638929055658No Hit
TGGGTTATCTCGTTTAGTTCTTGACCTT3070.10645638929055658No Hit
ACAGCCGAGGCTATCTGAACAGCAAACC3070.10645638929055658No Hit
GAACGTTGTTTCAGACCGATGTATGGCC3070.10645638929055658No Hit
CCACAAATCCGACAGCCTATAAAAAGAC3060.10610962580752546No Hit
ATTTACCCACTCCCTAGGAATCCGCCCC3060.10610962580752546No Hit
TGCTCGTTCCAAATGCCTAGTTATATAC3050.10576286232449435No Hit
AGGAGGTGTCTTCCGTTATTAGTCGCCC3050.10576286232449435No Hit
TCGACGGAGTTCCATGCGAGCGGCCCTG3040.1054160988414632No Hit
AGCCAATAGAGCAGCTTAAACCCATCTA3040.1054160988414632No Hit
CTCCGATGTAGCTGTTTCTGCCAATCCA3020.10472257187540093No Hit
CGTAATGGTATACCTGATTTCTCCATAC3020.10472257187540093No Hit
CGGAATTCACTTGTCCCTCATGCTGTCA3020.10472257187540093No Hit
AATGCCATCAACACGTTTACTAATGGTC3010.10437580839236982No Hit
CGAGAAGTCATTGGTGACCTAAAAATTG3010.10437580839236982No Hit
GCAGTTACAAAGTGTATAACCCGTGTTT2990.10368228142630756No Hit
CAGATTGTCGAAGGACAAATGGCGAATC2970.10298875446024529No Hit
TAGGGTTGTACTCGTACGACAATGGTCG2970.10298875446024529No Hit
TCAGGGCCATCACAGTTAAGTCCTCTCA2960.10264199097721417No Hit
TGCCGAGGTCCTACAACGACCTCAGTAG2960.10264199097721417No Hit
CTCCGATTCGCTATTTTTGTACCCCTCG2950.10229522749418306No Hit
AGGTGTTCATCAGCATTATATATTTCAC2950.10229522749418306No Hit
TGCAGGCGTGCAACAGTTGCTTTGGCCT2950.10229522749418306No Hit
TTGAGTGGTGATACAAGACTTAACACAT2950.10229522749418306No Hit
GCTTTCGTCGTCTAAGCTCCGAACCTTT2950.10229522749418306No Hit
GATGACTTCGACACTACTTCCACATCAC2950.10229522749418306No Hit
GTTTGGAGTGACACGATCTTATCAAGAT2940.10194846401115192No Hit
TTCGATTAGGTGGGTTCATATGTCTCCT2910.10090817356205853No Hit
CGAAGGAGTGTTACACTATGACTCTATA2890.10021464659599627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)