FastQCFastQC Report
Mon 30 Dec 2024
EGAF00008204522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008204522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38287458
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4683001.2231159352496057No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACTTGATCTCGTAT2742260.7162293197944872TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACTTGATCGCGTAT904430.23622095778727334TruSeq Adapter, Index 15 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACTTGATCTCGTATG416700.10883459539152482TruSeq Adapter, Index 15 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)