FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008205402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008205402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847164
Sequences flagged as poor quality0
Sequence length50-150
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCCGTTTTTCCCCCGGACTTTTTTCCACACTTGGCAAGGAGCCGTCA8598775.426062354122163No Hit
TCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGC489530.30890700695720696No Hit
TAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGCGCT278350.17564656994778372No Hit
TGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGCGCTCCAGAAG265320.16742427856492179No Hit
CGATGTCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTG249070.1571700778763948No Hit
CGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGCG223790.1412176967437202No Hit
GGGCCGTTTTTCCCCCGGACTTTTTTCCACACTTGGCAAGGAGCCGTCAT208350.13147462852028285No Hit
TGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGCGCTCCAGAAGCCGT200880.12676085134223383No Hit
GATGTCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGC193090.12184514528908769No Hit
CAGCCGATGTCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGT190760.12037485066728659No Hit
AGCCGATGTCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTG180320.11378692111724217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)