FastQCFastQC Report
Mon 4 Sep 2023
EGAF00008205403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008205403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15847164
Sequences flagged as poor quality0
Sequence length50-150
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCCGTTTTTCCCCCGGACTTTTTTCCACACTTGGCAAGGAGCCGTCA8520115.376425712512346No Hit
TCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGC455370.2873510995405866No Hit
TAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGCGCT266130.16793541103001142No Hit
TGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGCGCTCCAGAAG257660.16259060611728382No Hit
CGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGCG229290.1446883492844524No Hit
CGATGTCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTG229290.1446883492844524No Hit
CAGCCGATGTCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGT197460.1246027364896331No Hit
TGTCTGGCGCGGGATCGACAGCACCGTGTTGCGGGCGCTCCAGAAGCCGT195980.12366881544230879No Hit
GATGTCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTGTTGC189120.11933996518241372No Hit
GGGCCGTTTTTCCCCCGGACTTTTTTCCACACTTGGCAAGGAGCCGTCAT178410.11258165814400607No Hit
AGCCGATGTCGTAGGTCTTGCCTGTCTGGCGCGGGATCGACAGCACCGTG175670.11085264215098677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)