FastQCFastQC Report
Mon 5 Aug 2024
EGAF00008207553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49240097
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1186610.24098449684207568No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACATCTATCTCGTAT1130840.22965836155846728TruSeq Adapter, Index 23 (97% over 37bp)
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT684790.13907161880692476No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT613730.12464029061518705No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT606410.12315369728049072No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA588120.11943924480896127No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC518020.10520287967751159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)