FastQCFastQC Report
Mon 5 Aug 2024
EGAF00008207554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49240097
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1533360.3114047480450739No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1414770.2873207175038668No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1291350.2622557790655855No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1187150.2410941635634877No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA1186680.240998712898555No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1143480.23222537518559316Illumina Single End PCR Primer 1 (100% over 50bp)
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT822430.16702444757572268No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG816500.16582014450540178No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG765180.15539774424083688No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG759390.15422187328347464No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA720610.1463461779939223No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT717110.14563537516995548No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG704820.1431394418252263No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT684450.13900256938973943No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA683370.13878323594691538No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT672770.1366305188229016No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT644920.13097455920933707No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC587130.11923818915303924No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC582490.11829586769498038No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT563490.11443722379344623No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC538580.10937833855201383No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA534130.10847460353297028No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG527540.10713626335870136No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC516840.10496323758257423No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT514160.10441896570593677No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG501360.10181945823542955No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC493010.10012368578396587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)