FastQCFastQC Report
Sat 3 Aug 2024
EGAF00008207683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9003608
Total Bases909.3 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT149140.16564470598897685No Hit
GGATGACATAACTATTAGTGGCAGGTTAGTTGTTTGTAGGGCTCATGGTA124230.13797801947841354No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG122160.13567894115336873No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCATTATCTCGTAT118200.13128070435763087TruSeq Adapter, Index 1 (97% over 36bp)
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA117920.13096971791752818No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC107780.11970756612238115No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT97870.10870086747446135No Hit
GTTTTCTCGTGTGAATGAGGGTTTTATGTTGTTAATGTGGTGGGTGAGTG94980.1054910431462587No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCTACAACGT94530.1049912435103794No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA92550.10279212511251044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)