FastQCFastQC Report
Sat 3 Aug 2024
EGAF00008207684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9003608
Total Bases909.3 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT297060.32993439963179205No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT267640.2972586101038606No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC255200.2834419268364416No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC245070.27219088169986966No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA211090.23445045586169455No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA202910.2253652091472663No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC201820.22415458336258087No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA195820.21749058821752348No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT192800.2141363773278446No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT176000.1954771909216838No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC168950.1876469966262414No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC163330.18140505450703762No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA156600.17393027328599825No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA121960.13545680798186682No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG120910.13429060883148178No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA110240.1224398041318547No Hit
TTTTAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTT109180.12126249832289455No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG108520.12052945885693825No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG107770.11969645946380605Illumina Single End PCR Primer 1 (100% over 50bp)
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT103700.11517604942374213No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA103020.11442079664063562No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT102840.11422087678628391No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG102570.1139209970047563No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT101970.11325459749025057No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT95240.1057798162692112No Hit
TAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGA94810.10530222995048207No Hit
GTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCAC91090.10117055296054649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)