FastQCFastQC Report
Sun 4 Aug 2024
EGAF00008207694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31250887
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC3080080.9855976247970178No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1220520.3905553144779539No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA1198280.38343871647547156No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA1147860.3673047744212828No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC1074720.34390063872427046No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT956180.3059689153783059No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC900880.28827341764731346No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG861880.2757937718695792No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT851490.27246906623802386No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA841290.2692051588807703No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT836710.26773960047918No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG765720.2450234452545299No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA732990.23455014252875445No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG727180.23269099529878945No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC642120.20547256786663365No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG631970.20222466005524897No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT620440.19853516477788294No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG615540.1969672092827317No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT613340.19626322926450057No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT603540.1931273182741981No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA592000.18943462308765827No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA571590.18290360846397735No Hit
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT568990.18207163207879506No Hit
GTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCAC567920.18172924179720082No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG557830.1785005334408588No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC511190.16357615705435816No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG501740.16055224288513795No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT493320.15785791936081686No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA481940.15421642272105748No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG477050.15265166713508005Illumina Single End PCR Primer 1 (100% over 50bp)
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT469660.1502869342556581No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC467510.14959895378329582No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA466210.14918296559070465No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA445940.14269674969545665No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT445030.14240555796064283No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC444760.14231916041295084No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA439950.14078000410036362No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA438400.140284018178428No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT436270.13960243752441331No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG429090.1373049027376407No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT421190.13477697449035606No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT413460.13230344469902566No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC403480.12910993534359522No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG401650.12852435196479384No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG399640.12788117022086445No Hit
GCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC365970.1171070760327539No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG365950.11710067621440634No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG353770.11320318684074472No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC350390.11212161754000774No Hit
GCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAAC341280.10920650028269598No Hit
CTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCAT330360.10571219946493039No Hit
CGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCC327470.10478742571370854No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC322040.10304987503234708No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA319530.10224669782972881No Hit
CGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCA319260.10216030028203679No Hit
CCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCG316490.10127392544090029No Hit
TAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGA316200.10118112807486071No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC314730.10071074142631536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)