FastQCFastQC Report
Mon 5 Aug 2024
EGAF00008207698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37123226
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC3360800.9053092530266631No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA1376930.37090795934599No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1295850.3490671850555229No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG1188190.32006647267131366No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG1122240.3023013140075703No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG1012820.27282650489480625No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC974170.2624152329864867No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC874840.2356583988686759No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT781060.21039658568466005No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA766040.20635060110347092No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG711770.1917317207292276No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT703560.189520167239776No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT669990.18047731088887586No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC669770.18041804879780654No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA663870.1788287472645831No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA654040.1761808092863481No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC651250.1754292582223323No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT647020.17428980983495346No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG644670.17365678295307635No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT628460.16929024433383025No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC596210.16060296052934625No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT581650.1566808875931203No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT531370.14313680605236195No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA519490.13993665313461712No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC499340.13450878433894725No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG496320.13369527745244986No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT493660.13297874489679318No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT475540.12809770357780867No Hit
GTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCAC461590.12433994825772955No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG458090.12339714226344445No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG441310.11887706095370053No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA437520.11785613674846038No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC433390.11674362567520398No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA423500.11407952530849555No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT421860.11363775335688768No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA413520.11139118135907694No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG408030.10991232281375547No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA395110.10643202182913739No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT384410.10354972921803723No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG374470.1008721601942676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)