FastQCFastQC Report
Sat 3 Aug 2024
EGAF00008207705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7335017
Total Bases740.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGAATGATCTCGTAT207150.28241243339994987TruSeq Adapter, Index 10 (97% over 36bp)
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC83570.11393293294344102No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT78250.1066800526842678No Hit
GGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCG75720.10323084459108957No Hit
GTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCC73720.10050419787711468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)