FastQCFastQC Report
Sat 3 Aug 2024
EGAF00008207706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7335017
Total Bases740.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG193090.26324410700070633Illumina Single End PCR Primer 1 (100% over 50bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC121970.16628454985175903No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA120640.16447132978696571No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC105570.14392604679716486No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT95860.13068817700081675No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT94310.12857502579748623No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC91410.12462138806222263No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC90160.12291723386598831No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA88790.12104948086691551No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT84670.11543258863612722No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT80040.10912040149327533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)