FastQCFastQC Report
Sat 3 Aug 2024
EGAF00008207777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11224409
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA186200.16588846682261846No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATGCCATCTCGTAT140320.12501326350456404TruSeq Adapter, Index 22 (97% over 37bp)
GGATGACATAACTATTAGTGGCAGGTTAGTTGTTTGTAGGGCTCATGGTA128170.11418864013241142No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG127850.11390354717116953No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC120180.10707022525640326No Hit
GCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTAT119790.10672276820988971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)