FastQCFastQC Report
Sat 3 Aug 2024
EGAF00008207782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11927108
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG753740.6319553742617238Illumina Single End PCR Primer 1 (100% over 50bp)
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA307400.257732218069963No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT258610.21682540310693924No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC252280.21151816517465927No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC245050.205456343650112No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT206980.17353745769720538No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT203430.17056104463881772No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC199850.16755947879402114No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT199730.16745886764838552No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC198470.1664024506192113No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC196340.1646166027841787No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA175850.14743724966689326No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT172430.14456983201627754No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA167030.14004233046267378No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA166220.13936320522963322No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG145490.1219826298210765No Hit
CGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGC140380.11769827186942552No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA134070.11240780246141815No Hit
CTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTAC130370.10930562547098593No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT123000.1031264242765304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)