FastQCFastQC Report
Mon 5 Aug 2024
EGAF00008207826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38014732
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT894420.23528246891231538No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC755400.19871243601033411No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC666220.17525310976807623No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA537060.14127680815953142No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG505240.13290636903608843No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG469710.12355999247870536Illumina Single End PCR Primer 1 (100% over 50bp)
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT402880.10597996587217819No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA393440.10349671806182931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)