FastQCFastQC Report
Mon 5 Aug 2024
EGAF00008207872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008207872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39903395
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT4555311.1415845694332525No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG3949590.9897879616508821No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG3648960.9144485074515589No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG2515800.6304726703078773No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA2351700.5893483499336335No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT2066150.5177880227985614No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1771840.4440323937349191No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1654790.41469905004323565No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT1566730.392630752345759No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT1383340.34667225683428693No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC1193900.29919759960274056No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA1177460.29507764940802655No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT1167820.29266181486562737No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC1135280.2845071202588151No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA1082100.2711799334367414No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT993370.24894373022646318No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG960290.24065370878843767Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA901390.22589305997647566No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT858740.21520474636306008No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCGCTCATTCATCGACCTC841160.21079910619134037No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG764010.19146491169485705No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC698410.175025207754879No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA684420.1715192404054843No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT680950.17064964021231777No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA652210.1634472455288579No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG611620.15327517871599647No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG591420.14821295280764957No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA572260.14341135635200966No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT560410.14044168422260814No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG551960.138324069919364No Hit
GTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTA520270.13038238976909108No Hit
GCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTAT518450.1299262882268539No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC517320.12964310430227804No Hit
CGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTG495900.12427513999748643No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT495310.12412728290412384No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC484060.12130797392051478No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA480270.12035818004959228No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA478780.11998477823754095No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG476200.11933821671063327No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG468610.11743612291635838No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA465010.11653394404160348No Hit
GCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGG457690.1146995136629352No Hit
CAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCAC451680.1131933761525805No Hit
CGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCC445390.11161706917418933No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCGCTCATTCATCGACCT436370.10935660988244233No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG427800.10720892295003973No Hit
GCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAAT425090.10652978274154368No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC418860.10496851207773172No Hit
GGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGC418380.10484822156109776No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA415510.10412898451372371No Hit
GTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCAC405790.1016931015518855No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA405210.10154775051095276No Hit
AGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGG403970.1012370000096483No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC399800.10019197614639055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)