FastQCFastQC Report
Wed 8 May 2024
EGAF00008213203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008213203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793172
Total Bases30.1 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46200.5824713933421755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36100.45513457358555265No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18230.22983665585774588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17480.22038095142037287No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA17080.21533790905377395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14030.17688471100845718Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.10262591216028806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)