FastQCFastQC Report
Wed 8 May 2024
EGAF00008223060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008223060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183177
Total Bases6.9 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT75924.144625143986418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT51702.82240674320466No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA38232.0870524137855737No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA33731.8413883839128276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT29691.62083667709374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29071.5869896329779394No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA15930.8696506657495209No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.6807623227806985No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA11460.6256243960759266No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.5710323894375385No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA9930.5420986259191929No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA8030.43837381330625574No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7350.40125124879215185No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA7210.39360836786277753No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.36522052441081576No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT5980.3264601996975603No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA5780.3155417983698827No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA5520.30134787664390184No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG5290.2887917151170726No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA5260.28715395491792095No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA5010.2735059532583239No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC4970.27132227299278844No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA4820.2631334719970302No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4540.24784771013828155No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4290.23419970847868457No Hit
GAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAA4040.22055170681908753No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA4020.21945986668631978No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA4010.2189139466199359No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3870.21127106569056156No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAA3870.21127106569056156No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.19653122389819683No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA3600.19653122389819683No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG3450.18834242290243863No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3440.18779650283605476No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3170.17305666104368997No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3160.1725107409773061No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG3150.17196482091092222No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2850.15558721891940583No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT2840.15504129885302195No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2730.14903617812279926No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2670.14576065772449598Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAA2640.14412289752534435No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA2640.14412289752534435No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAA2600.14193921725980882No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2570.14030145706065716No Hit
CATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2530.13811777679512166No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA2530.13811777679512166No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2510.1370259366623539No Hit
GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.13320449619766672No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2420.132112656064899No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAA2410.1315667359985151No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2380.12992897579936347No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2290.12501569520190853No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2280.12446977513552465No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGC2140.11682689420615033No Hit
GTACATGGGTGGTATCAACTCAAAAAAAAAAAAAAAAA2140.11682689420615033No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.11573505407338257No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAA2080.11355137380784706No Hit
CATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1990.10863809321039213No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1950.10645441294485662No Hit
GTACATGGGGTATCAACGCAGAAAAAAAAAAAAAAAAA1920.10481665274570497No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1910.10427073267932109No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA1880.10263297248016945No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA1840.10044929221463395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)