FastQCFastQC Report
Wed 8 May 2024
EGAF00008225900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008225900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633633
Total Bases24 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT522328.243257532357058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT339245.353887818342795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT177182.7962558768245973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147842.3332118118847975No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67731.0689152869247656No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA64551.0187285068801657No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA55950.883003252671499No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT49690.7842078932126325No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA47800.7543799012993325No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAA40010.631438072196366No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36450.5752541297564995No Hit
GTACATGGGGTATCAACGCAGAAAAAAAAAAAAAAAAA34420.5432166569607327No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAA34340.5419540964564661No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAA28810.45467960159903287No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAA26270.4145933055885663No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAA25210.39786437890703297No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA24930.3934454171420996No Hit
GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23670.37356008919989964No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA22110.34894015936669964No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA21430.338208395080433No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAA20410.322110748651033No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19950.3148510257514997No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18900.29827991913299967No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAA18070.28518085390123304No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA17960.2834448332078664No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT15900.2509339002229998No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA15550.2454101980168331No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15540.24525237795379973No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAA14520.22915473152439977No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA14140.2231575691291331No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAGAAAA12820.2023253208087331No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAA12570.1983798192328998No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA12380.19538123803526647No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA11370.17944141166889982No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGA10750.16965656776083315No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA10640.1679205470674665No Hit
GTACATGGGGGTATCAACGCAGAAAAAAAAAAAAAAAA10640.1679205470674665No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA10270.1620812047352332No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAGAAAAA10120.15971390378973319No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGA9510.15008687994469985No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA9240.14582573824279985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9080.14330061723426651No Hit
TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA9060.14298497710819985No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA9050.14282715704516655No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA8840.13951293572146653No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAA8700.13730345483899986No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.1317797526328332No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8340.13162193256979987No Hit
ATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAA8100.12783425105699986No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAA7900.1246778497963332No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAA7660.12089016828353322No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA7580.11962760777926655No Hit
GAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA7340.11583992626646655No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7090.11189442469063321No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7060.11142096450153323No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6980.11015840399726656No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAA6950.10968494380816655No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6790.10715982279963324No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)