FastQCFastQC Report
Tue 7 May 2024
EGAF00008227635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008227635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112752
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1581614.027245636441037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT102779.11469419611182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT58725.2078898822193835No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39663.5174542358450402No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19381.7188165176670922No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT14731.306406981694338No Hit
GTATCAACGCAGAGTACATGGGGCACCCGAGAATTCCA12681.1245920249751666No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.964062721725557No Hit
GTACATGGGGCACCCGAGAATTCCAGTCATTGTATGTC10600.9401163615723003No Hit
TATCAACGCAGAGTACATGGGGCACCCGAGAATTCCAG7520.6669504753795941No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.646551724137931No Hit
GTATCAACGCAGAGTACATGGGGGCACCCGAGAATTCC6110.5418972612459203No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5840.5179509010926635No Hit
GACATACAATGACTGGAATTCTCGGGTGCCCCATGTAC5610.49755214985100044No Hit
GTACATGGGGGCACCCGAGAATTCCAGTCATTGTATGT5610.49755214985100044No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5570.4940045409394069No Hit
GTATCAACGCAGAGTACATGGGCCTTGGCACCCGAGAA5350.4744926919256421No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT5080.45054633177238546No Hit
ACGCAGAGTACATGGGGCACCCGAGAATTCCAGTCATT4700.4168440471122464No Hit
GGTATCAACGCAGAGTACATGGGGCACCCGAGAATTCC4650.4124095359727543No Hit
TATCAACGCAGAGTACATGGGGGCACCCGAGAATTCCA4420.39201078473109124No Hit
GTATCAACGCAGAGTACATGGGTGGCACCCGAGAATTC3950.3503263800198666No Hit
GTACATGGGGCACCCGAGAATTCCAAAGACGCATGTCA3830.33968355328508587No Hit
GTACATGGGCCTTGGCACCCGAGAATTCCAGTCATTGT3650.3237193131829147Illumina RNA RT Primer (100% over 22bp)
TATCAACGCAGAGTACATGGGCCTTGGCACCCGAGAAT3580.3175109975876259No Hit
CATACAATGACTGGAATTCTCGGGTGCCCCATGTACTC3360.29799914857386123No Hit
TATCAACGCAGAGTACATGGGTGGCACCCGAGAATTCC3280.29090393075067406No Hit
GTACATGGGCAATCCCATTTTACCATTTTTTCAACTAC2580.22882077479778629No Hit
GTATCAACGCAGAGTACATGGGTTGGCACCCGAGAATT2580.22882077479778629No Hit
GACATACAATGACTGGAATTCTCGGGTGCCAAGGCCCA2440.21640414360720875Illumina RNA RT Primer (100% over 22bp)
GACATACAATGACTGGAATTCTCGGGTGCCCCCATGTA2430.21551724137931033No Hit
ACGCAGAGTACATGGGCCTTGGCACCCGAGAATTCCAG2400.21285653469561516Illumina RNA RT Primer (100% over 22bp)
GGTATCAACGCAGAGTACATGGGGGCACCCGAGAATTC2340.20753512132822477No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.20753512132822477No Hit
ATCAACGCAGAGTACATGGGGCACCCGAGAATTCCAGT2290.20310061018873282No Hit
GTACATGGGTGGCACCCGAGAATTCCAGTCATTGTATG2290.20310061018873282No Hit
TATCAACGCAGAGTACATGGGGCACCCGAGAATTCCAA2230.1977791968213424No Hit
GTACATGGGTTGGCACCCGAGAATTCCAGTCATTGTAT2230.1977791968213424No Hit
GTATCAACGCAGAGTACATGGGCACCCGAGAATTCCAG2200.19511849013764723No Hit
ACGCAGAGTACATGGGGGCACCCGAGAATTCCAGTCAT2180.19334468568185043No Hit
TACAATGACTGGAATTCTCGGGTGCCCCATGTACTCTG2130.18891017454235845No Hit
GTACATGGGCACCCGAGAATTCCAGTCATTGTATGTCT2120.18802327231446006No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2070.18358876117496806No Hit
ATACATGGGGCACCCGAGAATTCCAGTCATTGTATGTC1900.16851142330069532No Hit
TATCAACGCAGAGTACATGGGTTGGCACCCGAGAATTC1880.16673761884489852No Hit
GACATACAATGACTGGAATTCTCGGGTGCCACCCATGT1840.16319000993330496No Hit
GTATCAACGCAGAGTACATGGGCAATCCCATTTTACCA1820.16141620547750815No Hit
CATTGTATGTCTGCAAAAAAAAAAAAAAAAAAAAAAAA1820.16141620547750815No Hit
TTGTATGTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAA1810.16052930324960976No Hit
GGTATCAACGCAGAGTACATGGGTGGCACCCGAGAATT1810.16052930324960976No Hit
CATACAATGACTGGAATTCTCGGGTGCCCCCATGTACT1800.15964240102171137No Hit
CATACAATGACTGGAATTCTCGGGTGCCAAGGCCCATG1760.15609479211011779Illumina RNA RT Primer (100% over 22bp)
ACGCAGAGTACATGGGTGGCACCCGAGAATTCCAGTCA1730.1534340854264226No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1680.14899957428693061No Hit
GGTATCAACGCAGAGTACATGGGCCTTGGCACCCGAGA1630.1445650631474386No Hit
ACGCAGAGTACATGGGGCACCCGAGAATTCCAAAGACG1580.14013055200794666No Hit
GTACATGGGGCACCCGAGAATTCCAGTCATTGTATGTA1540.13658294309635305No Hit
TATCAACGCAGAGTACATGGGCACCCGAGAATTCCAGT1520.13480913864055627No Hit
TATCAACGCAGAGTACATGGGCAATCCCATTTTACCAT1480.13126152972896268No Hit
ACGCAGAGTACATGGGCAATCCCATTTTACCATTTTTT1470.1303746275010643No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1440.1277139208173691No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1350.11973180076628352No Hit
CATACAATGACTGGAATTCTCGGGTGCCACCCATGTAC1340.11884489853838513No Hit
GACTGGAATTCTCGGGTGCCCCATGTACTCTGCGTTGA1330.11795799631048674No Hit
TACAATGACTGGAATTCTCGGGTGCCAAGGCCCATGTA1320.11707109408258833Illumina RNA RT Primer (100% over 22bp)
TTGTATGTCTGTAAAAAAAAAAAAAAAAAAAAAAAAAA1280.11352348517099475No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1270.11263658294309636No Hit
TTGTATGTCTGTCAAAAAAAAAAAAAAAAAAAAAAAAA1230.10908897403150276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT1210.10731516957570597Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1200.10642826734780758No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1200.10642826734780758No Hit
GTACATGGGGGCACCCGAGAATTCCAAAGACGCATGTC1170.10376756066411238No Hit
GGTATCAACGCAGAGTACATGGGCACCCGAGAATTCCA1160.102880658436214No Hit
GTATCAACGCAGAGTACATGGGCTTGGCACCCGAGAAT1160.102880658436214No Hit
ATTCCAGTCATTGTATGTCTGCAAAAAAAAAAAAAAAA1150.10199375620831559No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1140.1011068539804172No Hit
ATCAACGCAGAGTACATGGGGGCACCCGAGAATTCCAG1130.10021995175251881No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)