FastQCFastQC Report
Tue 7 May 2024
EGAF00008228074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008228074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78299
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48076.139286580926959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36884.710149554911301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19742.5211049949552358No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAA14681.874864302226082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13121.6756280412265803No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA11371.4521258253617542No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA9831.2554438754007076No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9291.1864774773624183No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7640.9757468166898683No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6790.8671885975555244No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGA6020.7688476225750009No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.7254243349212633No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAA5110.6526264703252915No Hit
GTACATGGGGTATCAACGCAGAAAAAAAAAAAAAAAAA4560.5823829167677749No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAA4130.527465229440989No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAA4060.518525140806396No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAA3670.4687160755565205No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAA3480.4444501206911966No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAA3210.409966921672052No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.3933638999220935No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA2710.3461091457106732No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA2470.31545741324921134No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2420.3090716356530735No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAA2380.3039630135761632No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2350.30013154701848044No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2210.2822513697492944No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA2180.27841990319161164No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2100.26820265903779106No Hit
GTACATGGGGGTATCAACGCAGAAAAAAAAAAAAAAAA1980.2528767928070601No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAAA1940.2477681707301498No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAG1660.21200781619177766No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1660.21200781619177766No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA1570.20051341651872948No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAGAAAA1520.1941276389225916No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1480.1890190168456813No Hit
TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.18646470580722616No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAA1430.18263323924954342No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1430.18263323924954342No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1380.17624746165340555No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAA1380.17624746165340555No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1330.16986168405726765No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA1260.16092159542267462No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1250.15964443990344704No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAAA1170.14942719574962643No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1130.14431857367271614No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAGAAAAA1120.14304141815348856No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGA1080.13793279607657827No Hit
GTATCAACGCAGAGTACATGGGGCACCCGAGAATTCCA1080.13793279607657827No Hit
GAGTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAA1030.13154701848044037No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1010.1289927074419852No Hit
GTATCAACGCAGAGTACATGGGGGCACCCGAGAATTCC1010.1289927074419852No Hit
GTACATGGGAGTGGTATCAACGCAGAAAAAAAAAAAAA990.12643839640353005No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.12260692984584733No Hit
ACGCAGAGTACATGGGGTATCAACGCAGAAAAAAAAAA910.11622115224970944No Hit
GTATCAACGCAGAGTACATGGGGGTATCAACGCAGAAA900.11494399673048186No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA810.10344959705743369No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT790.10089528601897853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)