FastQCFastQC Report
Wed 8 May 2024
EGAF00008230571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008230571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210382
Total Bases7.9 Mbp
Sequences flagged as poor quality0
Sequence length38
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT136056.466807996881863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT105034.992347254042646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT52242.48310216653516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49952.3742525501231095No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22991.0927740966432489No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT18620.8850567063722182No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA18060.8584384595640312No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.6526223726364423No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA10560.5019440826686694No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAA9840.46772062248671464No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAA9820.4667699708149937No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAA8610.40925554467587527No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA7110.33795666929680296No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6880.32702417507201187No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAA6790.32274624254926754No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.31989428753410465No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGA6710.31894363586238367No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6620.3146657033396393No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6470.30753581580173206No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAA6380.3032578832789878No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAA6280.29850462492038293No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT6240.296603321576941No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA5090.2419408504529855No Hit
GTACATGGGGTATCAACGCAGAAAAAAAAAAAAAAAAA4810.22863172704889204No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAGAAAAAAAA4670.22197716534684528No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAGAAAAAAA4100.19488359270279776No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAA3800.1806238176269833No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3510.1668393683870293No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAA3340.1587588291774011No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3240.1540055708187963No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAG3130.14877698662433098No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGA3050.14497437993744713No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.14069644741470277No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA2910.13831981823540035No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2860.13594318905609795No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2850.13546786322023746No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2570.12215873981614397No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG2570.12215873981614397No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2540.12073276230856252No Hit
ATCAACGCAGAGTACATGGGTGGTATCAACGCAGAAAA2390.11360287477065528No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2390.11360287477065528No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2360.11217689726307382No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAAA2220.1055223355610271No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2130.10124440303828275No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAA2110.10029375136656178No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)