FastQCFastQC Report
Wed 8 May 2024
EGAF00008243230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008243230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104219
Total Bases83.6 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27377224.793270175572054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160271.451433094340887No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54040.48939567241643184No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG46930.42500627140087244No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT39480.3575377710399839No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.2512182818806777No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.24261491606284621No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT23760.21517470719123652No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG19870.1799461882108531No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC18950.17161450762937425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18140.16427900624785483No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG16980.1537738437755554No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT16160.1463477806485851No Hit
GCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAG16040.14526103970317483No Hit
GCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC15970.1446271074850188No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15690.14209137861239482No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT14880.1347558772308754No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.12787318457661026No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAA13860.12551857919488799No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT12960.11736802210431084No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT11870.1074967918501674No Hit
CCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACC11520.1043271307593874No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC11250.10188196363221426No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)