FastQCFastQC Report
Wed 8 May 2024
EGAF00008243262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008243262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147602
Total Bases86.9 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31377527.341796197636466No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136211.1869097474559995No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54370.4737705232301791No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44700.3895078607391761No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31360.27326547008457636No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26900.23440182223453776No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG26670.23239764308532052No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT26650.23222336663756252No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT20960.18264171725040562No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16260.14168675202727077No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG14950.13027164469912042No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG14820.1291388477886933No Hit
GCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAG14450.1259147335051699No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC14240.1240848308037107No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.12208065165449344No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT11810.10291024240111118No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT11690.10186458371456307No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)