FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65523151
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG27019154.123603579443241TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3738800.5706074788741463TruSeq Adapter, Index 9 (100% over 50bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2178140.3324229629921186No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1924030.29364125055585316No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1509080.2303124890925957No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT984560.15026139386977894No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC964630.1472197208586626No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT860800.1313734133451549No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG853570.13026998655788088TruSeq Adapter, Index 9 (97% over 49bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC846780.12923371160828329No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)