FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49493373
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT7890751.5943043526251484TruSeq Adapter, Index 13 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3406520.6882780044108128No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2210910.44670828961283365No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1975850.3992150625902987No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1885500.38096009338462344No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1516660.3064369850080737Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1437460.2904348426606528No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1434140.289764045784473No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1392030.2812558360085905No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1338410.2704220623637835No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1210530.24458425979575082Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1172210.23684180910442293No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1067750.21573595317498367No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1054190.2129961924397434No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA972510.19649297290770623No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG950490.19204389242171876No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC870160.17581343667969448No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG820880.16585654810796588No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG807390.16313093068035595No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC791790.15997899355131848No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA745200.15056561208709698No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC705370.1425180700454584No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG622790.12583300798674604No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG606170.12247498266080997TruSeq Adapter, Index 2 (97% over 35bp)
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA604460.12212948186012701No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG565780.11431429415812902Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG501340.10129436924818198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)