FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63724004
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT5437360.8532671613039255TruSeq Adapter, Index 25 (97% over 44bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1783340.2798537267055598No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1596730.2505696283617081No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA1478100.23195340958173313TruSeq Adapter, Index 25 (97% over 44bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1460240.22915069806348012No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1295760.20333938840377952No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1062300.1667032724434579No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA771490.12106740813085128No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG686470.10772549697285186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)