FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51289906
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG16307223.179420917636308Illumina Single End PCR Primer 1 (100% over 50bp)
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG1270370.24768421295215476No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1269250.24746584639870464No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT1155260.22524120048104593No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1037500.20228151714686315No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG917120.17881101205371674No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA915020.1784015747659978No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT810020.15792971038005021No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT782010.15246859684242745No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC752490.14671307839792103No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG752310.1466779837732594No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT584690.11399708940780667No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC547310.10670910568640933No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT541430.10556268128079627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)