FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35488510
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT3717121.047415064763215TruSeq Adapter, Index 14 (97% over 44bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1602790.4516363183464169No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1267730.3572226616445717No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC925370.2607520011406509Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC914610.2577200338926599No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT832280.23452097594404497No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT715760.20168781388680448No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC639810.18028652090493513No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA596970.1682150081815213No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC557450.15707900951603773Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC555470.15652108245739255No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT502960.1417247441495853No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC488200.137565651530594No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA487840.13746421024720396No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA435200.1226312403648392No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG382690.10783490205703199Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)