FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39779885
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT4825621.2130804299710771TruSeq Adapter, Index 13 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1703590.42825412893978954No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1161630.29201441884510226Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC969160.24363066911832448No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC752030.18904780644790703Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC669620.16833130613625452No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG485050.12193348472475474Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT403060.10132256541214235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)