FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40721106
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT4822651.1843121353334558TruSeq Adapter, Index 13 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1784350.4381880000999973No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1377330.3382349192578414Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1003490.24642994716302644No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC845800.20770555691684797Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC699830.17185928103229808No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG616610.15142270448155312Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC474000.11640155353344282Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT460290.11303474910529197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)