FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40721106
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1381380.3392294894937284Illumina Single End PCR Primer 1 (100% over 32bp)
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC884880.21730254576091326No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA853800.20967014009884702Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG610130.1498313921041339Illumina Single End PCR Primer 1 (100% over 30bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA519980.12769299537198228No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT513190.12602555539626062No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG455620.11188792367280004Illumina Single End PCR Primer 1 (100% over 28bp)
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT453150.11128135861535784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)