FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56592981
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6514861.1511780939760003Illumina Single End PCR Primer 1 (100% over 50bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1421120.25111241268594775Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1404950.24825516789794125Illumina Single End PCR Primer 1 (100% over 34bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1271400.22465683509409057No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1004130.17743013042553812No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT893370.15785879877930445No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG891560.15753897113141999No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA837580.14800068580942927No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT806940.14258658684192657No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA720660.12734087995824075Illumina Single End PCR Primer 1 (100% over 36bp)
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT681720.12046016801977616No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA667750.11799166401925355No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT660340.11668231436686469No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC649600.11478455252251157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)