FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56347945
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT593369410.530453240131472TruSeq Adapter, Index 22 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA6220351.1039178092475244TruSeq Adapter, Index 22 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT928790.16483121079215932TruSeq Adapter, Index 22 (97% over 40bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA668740.11868045942048108No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT605320.10742539057990491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)