FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95679835
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT18383701.9213766411700022TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA15612401.6317335831526047TruSeq Adapter, Index 21 (97% over 40bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1381300.14436688775644316No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1369000.1430813504224793No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1057250.11049872734416818No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT978690.10228801084366419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)