FastQCFastQC Report
Fri 29 Sep 2023
EGAF00008245138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008245138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49448919
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT7217281.459542523063042TruSeq Adapter, Index 22 (97% over 40bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2079490.4205329544211068No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1216500.246011444658679No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1190510.24075551580814133Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1078630.21813014759736205No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC939960.1900870674240624Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC840970.1700684296051042No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT785610.15887303825590202No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA782860.1583169088084615No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT738790.14940468162711504No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT693630.14027202495569216No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT672380.1359746610436519No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA634370.12828794093557436No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC591790.11967703480029564No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG518300.10481523367578571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)